pet30b vector Search Results


dna pbr322  (Merck KGaA)
90
Merck KGaA dna pbr322
( a ) Gel images obtained after <t>pBR322</t> plasmid was irradiated with laser light using a 10 cm focal length lens. The negative and positive signs above the image panel indicate, respectively, no laser exposure and laser exposure for 180 seconds. Also shown are schematic depictions of single strand breaks (SSBs) and double strand breaks (DSBs) induced upon laser irradiation. Linear DNA results from DSBs. ( b ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states on the focal length of the external lens. The bars marked “Control” pertain to DNA prior to irradiation. ( c ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states for focal lengths of 8.5 cm to 12.5 cm. In each case irradiation was for 180 seconds using 820 nm laser light with the laser energy kept fixed (230 μ J) for different lenses.
Dna Pbr322, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
dna pbr322 - by Bioz Stars, 2026-04
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( a ) Gel images obtained after pBR322 plasmid was irradiated with laser light using a 10 cm focal length lens. The negative and positive signs above the image panel indicate, respectively, no laser exposure and laser exposure for 180 seconds. Also shown are schematic depictions of single strand breaks (SSBs) and double strand breaks (DSBs) induced upon laser irradiation. Linear DNA results from DSBs. ( b ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states on the focal length of the external lens. The bars marked “Control” pertain to DNA prior to irradiation. ( c ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states for focal lengths of 8.5 cm to 12.5 cm. In each case irradiation was for 180 seconds using 820 nm laser light with the laser energy kept fixed (230 μ J) for different lenses.

Journal: Scientific Reports

Article Title: Optical control of filamentation-induced damage to DNA by intense, ultrashort, near-infrared laser pulses

doi: 10.1038/srep27515

Figure Lengend Snippet: ( a ) Gel images obtained after pBR322 plasmid was irradiated with laser light using a 10 cm focal length lens. The negative and positive signs above the image panel indicate, respectively, no laser exposure and laser exposure for 180 seconds. Also shown are schematic depictions of single strand breaks (SSBs) and double strand breaks (DSBs) induced upon laser irradiation. Linear DNA results from DSBs. ( b ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states on the focal length of the external lens. The bars marked “Control” pertain to DNA prior to irradiation. ( c ) Dependence of the percentage of DNA in supercoiled, relaxed, and linear states for focal lengths of 8.5 cm to 12.5 cm. In each case irradiation was for 180 seconds using 820 nm laser light with the laser energy kept fixed (230 μ J) for different lenses.

Article Snippet: Our DNA (pBR322), obtained from a commercial source (Merck-Millipore, India).

Techniques: Plasmid Preparation, Irradiation, Control